In vitro chronic wound healing using collagen and plant extract along with zinc nanoparticles


  • J Sofia Bobby Jerusalem College of Engineering
  • S Purnima Jerusalem College of Engineering
  • V Mythily Jerusalem College of Engineering
  • B Ghiri Rajan Jerusalem College of Engineering
  • S Shubhankar Jerusalem College of Engineering
  • M Sowmiya Jerusalem College of Engineering



Type-I collagen, Cassia fistula, Zinc nanoparticles, Anti-Bacterial culture, MIT assay, Biofilm


INTRODUCTION: While the use of zinc nanoparticles (ZnNPs) as an antibacterial agent in the biomedical industry has recently attracted significant attention, collagen has aroused significant interest as a biomaterial in medical and tissue engineering applications.

OBJECTIVES: In order to create biofilm loaded with biosynthesized ZnNPs for use in chronic wound healing applications, type-I collagen was extracted from the study's subject. by the acid soluble collagen technique, collagen was isolated from the fish skin of the trevally and identified by SDS-PAGE. Aqueous extract from Cassia fistula leaves was also used to greenly manufacture stable ZnNPs, which were then characterized by UV-Vis, FTIR, and XRD measurements.

METHODS: Collagen and ZnNPs were then added to polyvinyl alcohol (PVA), creating a thin biofilm that had a high biocompatibility due to the production method's absence of a chemical reducer and crosslinking agent. When tested against the harmful bacteria, both ZnNPs alone and PVA/Collagen/ZnNPs biofilms showed potent antibacterial activity.

RESULTS: By using the MTT test, the cytotoxic effects of collagen and ZnNPs on the Vero cell line were evaluated. With 97.76% wound closure, the PVA/Collagen/ZnNPs biofilm demonstrated strong in vitro wound scratch healing efficacy.

CONCLUSION: The findings show that the PVA/Collagen/ZnNPs film dramatically increased cell migration by 40.0% at 24 hours, 79.20% at 48 hours, and 97.76% at 74 hours.


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How to Cite

Bobby JS, Purnima S, Mythily V, Ghiri Rajan B, Shubhankar S, Sowmiya M. In vitro chronic wound healing using collagen and plant extract along with zinc nanoparticles. EAI Endorsed Trans Perv Health Tech [Internet]. 2024 Apr. 15 [cited 2024 May 20];10. Available from: