In vitro chronic wound healing using collagen and plant extract along with zinc nanoparticles

Authors

  • J Sofia Bobby Jerusalem College of Engineering
  • S Purnima Jerusalem College of Engineering
  • V Mythily Jerusalem College of Engineering
  • B Ghiri Rajan Jerusalem College of Engineering
  • S Shubhankar Jerusalem College of Engineering
  • M Sowmiya Jerusalem College of Engineering

DOI:

https://doi.org/10.4108/eetpht.10.5784

Keywords:

Type-I collagen, Cassia fistula, Zinc nanoparticles, Anti-Bacterial culture, MIT assay, Biofilm

Abstract

INTRODUCTION: While the use of zinc nanoparticles (ZnNPs) as an antibacterial agent in the biomedical industry has recently attracted significant attention, collagen has aroused significant interest as a biomaterial in medical and tissue engineering applications.

OBJECTIVES: In order to create biofilm loaded with biosynthesized ZnNPs for use in chronic wound healing applications, type-I collagen was extracted from the study's subject. by the acid soluble collagen technique, collagen was isolated from the fish skin of the trevally and identified by SDS-PAGE. Aqueous extract from Cassia fistula leaves was also used to greenly manufacture stable ZnNPs, which were then characterized by UV-Vis, FTIR, and XRD measurements.

METHODS: Collagen and ZnNPs were then added to polyvinyl alcohol (PVA), creating a thin biofilm that had a high biocompatibility due to the production method's absence of a chemical reducer and crosslinking agent. When tested against the harmful bacteria, both ZnNPs alone and PVA/Collagen/ZnNPs biofilms showed potent antibacterial activity.

RESULTS: By using the MTT test, the cytotoxic effects of collagen and ZnNPs on the Vero cell line were evaluated. With 97.76% wound closure, the PVA/Collagen/ZnNPs biofilm demonstrated strong in vitro wound scratch healing efficacy.

CONCLUSION: The findings show that the PVA/Collagen/ZnNPs film dramatically increased cell migration by 40.0% at 24 hours, 79.20% at 48 hours, and 97.76% at 74 hours.

Downloads

Download data is not yet available.

References

Mohammad H, You HW, Umapathi M, Ravikumar KK, Mishra S. Strategies of Artificial intelligence tools in the domain of nanomedicine. Journal of Drug Delivery Science and Technology. 2023 Nov 10:105157. DOI: https://doi.org/10.1016/j.jddst.2023.105157

Zayed HS, Saleh S, Omar AE, Saleh AK, Salama A, Tolba E. Development of collagen–chitosan dressing gel functionalized with propolis–zinc oxide nanoarchitectonics to accelerate wound healing. International Journal of Biological Macromolecules. 2024 Mar 1;261:129665. DOI: https://doi.org/10.1016/j.ijbiomac.2024.129665

Sujogya K. Panda, L. P. Padhi, et al. Antibacterial activities and phytochemical analysis of Cassia fistula (Linn.) leaf- Pub med (2011) DOI: https://doi.org/10.4103/2231-4040.79814

Wang L, Yang Y, Han W, Ding H. Novel design and development of Centella Asiatica extract-loaded poloxamer/ZnO nanocomposite wound closure material to improve anti-bacterial action and enhanced wound healing efficacy in diabetic foot ulcer. Regenerative Therapy. 2024 Dec 1;27:92-103. DOI: https://doi.org/10.1016/j.reth.2024.03.006

Khan N, Arora I, Chandra A, Garg S. Biofabrication of Nanoparticles in Wound‐Healing Materials. Green Synthesis of Nanomaterials: Biological and Environmental Applications. 2024 May 5:208-27. DOI: https://doi.org/10.1002/9781119900931.ch10

Mathew-Steiner, S.S.; Roy, S.; Sen, C.K. Collagen in Wound Healing- BioengineeringMDPI (2020). DOI: https://doi.org/10.3390/bioengineering8050063

Shah, R. K., Boruah, F. & Parween, N. Synthesis and characterization of ZnO nanoparticles using leaf extract of Camelia sinensis and evaluation of theirantimicrobial efficacy. Int. J. Curr. Microbiol. Appl. Res. 4, 444–450 (2019).

Aminzai MT, Patan A. Recent Applications and Evaluation of Metal Nanoparticle–Polymer Hybrids as Chronic Wound Dressings. Journal of Nanomaterials. 2024 Jan 8;2024. DOI: https://doi.org/10.1155/2024/3280349

Prasath Alias Surendhar S, Kanna RK, Indumathi R. Ensemble Feature Extraction with Classification Integrated with Mask RCNN Architecture in Breast Cancer Detection Based on Deep Learning Techniques. SN Computer Science. 2023 Aug 14;4(5):618. DOI: https://doi.org/10.1007/s42979-023-01893-z

Sharma Y, Bhardwaj R, Kaur A, Singh G, Kulkarni S, Bhatia A, Bala K. Nano-gel formulation of polyphenolic fraction of tobacco stem for wound healing and its inhibitory efficacies against the receptors of chronic wound development. Journal of Biomolecular Structure and Dynamics. 2024 Feb 12:1-5. DOI: https://doi.org/10.1080/07391102.2024.2317986

Liang, C.C., Park, A.Y., Guan, J.L. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2007, 2, (2), pp. 329–333. DOI: https://doi.org/10.1038/nprot.2007.30

Ya-Hui Chan and Sheng-Wei Feng, Physiochemical Characterization, Biocompatibility, and Antibacterial Properties of CMC/PVA/Calendula officinalis Films for Biomedical applications (2023).

Pathak D, Mazumder A. A critical overview of challenging roles of medicinal plants in improvement of wound healing technology. DARU Journal of Pharmaceutical Sciences. 2024 Jan 15:1-41. DOI: https://doi.org/10.1007/s40199-023-00502-x

Lin Y, Zhang Y, Cai X, He H, Yang C, Ban J, Guo B. Design and Self-Assembly of Peptide-Copolymer Conjugates into Nanoparticle Hydrogel for Wound Healing in Diabetes. International Journal of Nanomedicine. 2024 Dec 31:2487-506. DOI: https://doi.org/10.2147/IJN.S452915

Sadeghianmaryan A, Ahmadian N, Wheatley S, Sardroud HA, Nasrollah SA, Naseri E, Ahmadi A. Advancements in 3D-printable polysaccharides, proteins, and synthetic polymers for wound dressing and skin scaffolding–A review. International Journal of Biological Macromolecules. 2024 Mar 28:131207. DOI: https://doi.org/10.1016/j.ijbiomac.2024.131207

Cai J, Liu S, Zhong Q, Shang Y, Chen Z, Yao Y, Zhou B, Yin F, Zhao J, Zheng L. Multifunctional PDA/ZIF8 based hydrogel dressing modulates the microenvironment to accelerate chronic wound healing by ROS scavenging and macrophage polarization. Chemical Engineering Journal. 2024 Mar 24:150632. DOI: https://doi.org/10.1016/j.cej.2024.150632

Indrakumar S, Dash TK, Mishra V, Tandon B, Chatterjee K. Silk Fibroin and Its Nanocomposites for Wound Care: A Comprehensive Review. ACS Polymers Au. 2024 Feb 29. DOI: https://doi.org/10.1021/acspolymersau.3c00050

Ravikumar KK, Ishaque M, Panigrahi BS, Pattnaik CR. Detection of Covid-19 using AI application. EAI endorsed transactions on pervasive health and technology. 2023 Jun 28;9. DOI: https://doi.org/10.4108/eetpht.9.3349

Pooja K, Kishore Kanna R. A Systematic Review on Detection of Gastric Cancer in Endoscopic Imaging System in Artificial Intelligence Applications. In International Conference on Data & Information Sciences 2023 Jun 16 (pp. 337-346). Singapore: Springer Nature Singapore. DOI: https://doi.org/10.1007/978-981-99-6906-7_29

Takallu S, Kakian F, Bazargani A, Khorshidi H, Mirzaei E. Development of antibacterial collagen membranes with optimal silver nanoparticle content for periodontal regeneration. Scientific Reports. 2024 Mar 27;14(1):7262. DOI: https://doi.org/10.1038/s41598-024-57951-w

Alven S, Aderibigbe BA. Chitosan-Based Scaffolds Incorporated with Silver Nanoparticles for the Treatment of Infected Wounds. Pharmaceutics. 2024 Feb 26;16(3):327. DOI: https://doi.org/10.3390/pharmaceutics16030327

Geetha S, Ramachandran V, Kanna RK, Vasuki R. Patient Monitoring System in Hospital. Indian Journal of Public Health Research & Development. 2019 May 1;10(5). DOI: https://doi.org/10.5958/0976-5506.2019.01139.2

Zheng Q, Chen C, Liu Y, Gao J, Li L, Yin C, Yuan X. Metal Nanoparticles: Advanced and Promising Technology in Diabetic Wound Therapy. International Journal of Nanomedicine. 2024 Dec 31:965-92. DOI: https://doi.org/10.2147/IJN.S434693

Amiri Z, Molavi AM, Amani A, Moqadam KH, Vatanchian M, Hashemi SA, Oroojalian F. Fabrication, characterization and wound-healing properties of core–shell SF@ chitosan/ZnO/Astragalus arbusculinus gum nanofibers. Nanomedicine. 2024 Jan(0). DOI: https://doi.org/10.2217/nnm-2023-0311

Sharma P, Sharma S, Soleimani MZ, Paiva-Santos AC, Nejaddehbashi F, Kumar A, Makvandi P, Xu Y. Accelerated in-vivo infected dermal wound healing with antimicrobial Bio-nanocomposite hydrogel. Alexandria Engineering Journal. 2024 Feb 1;88:230-44. DOI: https://doi.org/10.1016/j.aej.2023.12.059

Downloads

Published

15-04-2024

How to Cite

1.
Bobby JS, Purnima S, Mythily V, Ghiri Rajan B, Shubhankar S, Sowmiya M. In vitro chronic wound healing using collagen and plant extract along with zinc nanoparticles. EAI Endorsed Trans Perv Health Tech [Internet]. 2024 Apr. 15 [cited 2024 May 20];10. Available from: https://publications.eai.eu/index.php/phat/article/view/5784